Anti-PMS2 antibody

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Product name Anti-PMS2 antibody
Short Description Rabbit polyclonal against PMS2
Description The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.
Applications IF, WB
Dilution range WB 1:500 - 1:2000 IF 1:50 - 1:100
Protein Name Mismatch Repair Endonuclease Pms2
Dna Mismatch Repair Protein Pms2
Pms1 Protein Homolog 2
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 390-670 of human PMS2 (NP_000526. 2).
Storage Instruction Store at -20°C. Avoid freeze / thaw cycles.
Note For Research Use Only (RUO).
Validated Application
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Gene ID 5395
Gene Symbol PMS2
Molecular Weight Calculated: 20kDa/51kDa/62kDa/95kDa. Observed: 96kDa, 115kDa
Alternative Names Anti-Mismatch Repair Endonuclease Pms2 antibody
Anti-Dna Mismatch Repair Protein Pms2 antibody
Anti-Pms1 Protein Homolog 2 antibody
Anti-PMS2 PMSL2 antibody
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
Cellular Localization Nucleus
Swiss-Prot Key PMS2_HUMAN
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