• Western blot analysis of extracts of various cell lines, using POMGNT2 antibody (STJ116729) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-POMGNT2 antibody (160-340) (STJ116729)

SKU:
STJ116729

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POMGNT2 (160-340) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POMGNT2
Gene ID: 84892
Uniprot ID: PMGT2_HUMAN
Immunogen Region: 160-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-340 of human POMGNT2 (NP_116195.2).
Immunogen Sequence: NPDNLMHVFHDDLLPLFYTL RQFPGLAHEARLFFMEGWGE GAHFDLYKLLSPKQPLLRAQ LKTLGRLLCFSHAFVGLSKI TTWYQYGFVQPQGPKANILV SGNEIRQFARFMTEKLNVSH TGVPLGEEYILVFSRTQNRL ILNEAELLLALAQEFQMKTV TVSLEDHTFADVVRLVSNAS M
Tissue Specificity Highly expressed in the brain, muscle, heart, and kidney in both fetus and adult. In the brain, highest expression in the cortex and cerebellum. Highly expressed in the pancreas.
Function O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein. Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity.
Protein Name Protein O-Linked-Mannose Beta-1 -4-N-Acetylglucosaminyltransferase 2
Pomgnt2
Extracellular O-Linked N-Acetylglucosamine Transferase-Like
Glycosyltransferase-Like Domain-Containing Protein 2
Database Links Reactome: R-HSA-5173105
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Protein O-Linked-Mannose Beta-1 -4-N-Acetylglucosaminyltransferase 2 antibody
Anti-Pomgnt2 antibody
Anti-Extracellular O-Linked N-Acetylglucosamine Transferase-Like antibody
Anti-Glycosyltransferase-Like Domain-Containing Protein 2 antibody
Anti-POMGNT2 antibody
Anti-AGO61 antibody
Anti-C3orf39 antibody
Anti-EOGTL antibody
Anti-GTDC2 antibody

Information sourced from Uniprot.org

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