• Western blot analysis of extracts of various cell lines, using PPIA antibody (STJ25073) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PPIA antibody (100-165) (STJ25073)

SKU:
STJ25073

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPIA (100-165) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPIA
Gene ID: 5478
Uniprot ID: PPIA_HUMAN
Immunogen Region: 100-165
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-165 of human PPIA (NP_066953.1).
Immunogen Sequence: MANAGPNTNGSQFFICTAKT EWLDGKHVVFGKVKEGMNIV EAMERFGSRNGKTSKKITIA DCGQLE
Post Translational Modifications Acetylation at Lys-125 markedly inhibits catalysis of cis to trans isomerization and stabilizes cis rather than trans forms of the HIV-1 capsid. PPIA acetylation also antagonizes the immunosuppressive effects of cyclosporine by inhibiting the sequential steps of cyclosporine binding and calcineurin inhibition. Acetylation at Lys-125 favors its interaction with TARDBP.
Function Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2. Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1. Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates. Plays an important role in platelet activation and aggregation. Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton. Binds heparan sulfate glycosaminoglycans. Inhibits replication of influenza A virus (IAV). Inhibits ITCH/AIP4-mediated ubiquitination of matrix protein 1 (M1) of IAV by impairing the interaction of ITCH/AIP4 with M1, followed by the suppression of the nuclear export of M1, and finally reduction of the replication of IAV. (Microbial infection) May act as a mediator between human SARS coronavirus nucleoprotein and BSG/CD147 in the process of invasion of host cells by the virus. (Microbial infection) Stimulates RNA-binding ability of HCV NS5A in a peptidyl-prolyl cis-trans isomerase activity-dependent manner.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase A
Ppiase A
Cyclophilin A
Cyclosporin A-Binding Protein
Rotamase A Cleaved Into - Peptidyl-Prolyl Cis-Trans Isomerase A - N-Terminally Processed
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-162585
Reactome: R-HSA-162588
Reactome: R-HSA-162592
Reactome: R-HSA-162594
Reactome: R-HSA-164516
Reactome: R-HSA-164525
Reactome: R-HSA-173107
Reactome: R-HSA-175474
Reactome: R-HSA-180689
Reactome: R-HSA-2025928
Reactome: R-HSA-210991
Reactome: R-HSA-6798695
Reactome: R-HSA-8950505
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Secreted
Nucleus
Secretion Occurs In Response To Oxidative Stress In Vascular Smooth Muscle Through A Vesicular Secretory Pathway That Includes Rho Gtpase Signaling
Actin Remodeling
And Myosin Ii Activation
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase A antibody
Anti-Ppiase A antibody
Anti-Cyclophilin A antibody
Anti-Cyclosporin A-Binding Protein antibody
Anti-Rotamase A Cleaved Into - Peptidyl-Prolyl Cis-Trans Isomerase A - N-Terminally Processed antibody
Anti-PPIA antibody
Anti-CYPA antibody

Information sourced from Uniprot.org

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