• Western blot analysis of extracts of various cell lines, using PPIF antibody (STJ25075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PPIF antibody (30-207) (STJ25075)

SKU:
STJ25075

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPIF (30-207) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPIF
Gene ID: 10105
Uniprot ID: PPIF_HUMAN
Immunogen Region: 30-207
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human PPIF (NP_005720.1).
Immunogen Sequence: CSKGSGDPSSSSSSGNPLVY LDVDANGKPLGRVVLELKAD VVPKTAENFRALCTGEKGFG YKGSTFHRVIPSFMCQAGDF TNHNGTGGKSIYGSRFPDEN FTLKHVGPGVLSMANAGPNT NGSQFFICTIKTDWLDGKHV VFGHVKEGMDVVKKIESFGS KSGRTSKKIVITDCGQLS
Post Translational Modifications Acetylated at Lys-167.deacetylated at Lys-167 by SIRT3.
Function PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis.the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase F - Mitochondrial
Ppiase F
Cyclophilin D
Cyp-D
Cypd
Cyclophilin F
Mitochondrial Cyclophilin
Cyp-M
Rotamase F
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase F - Mitochondrial antibody
Anti-Ppiase F antibody
Anti-Cyclophilin D antibody
Anti-Cyp-D antibody
Anti-Cypd antibody
Anti-Cyclophilin F antibody
Anti-Mitochondrial Cyclophilin antibody
Anti-Cyp-M antibody
Anti-Rotamase F antibody
Anti-PPIF antibody
Anti-CYP3 antibody

Information sourced from Uniprot.org

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