• Western blot analysis of extracts of various cell lines, using PPME1 antibody (STJ115061) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PPME1 antibody (1-160) (STJ115061)

SKU:
STJ115061

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPME1 (1-160) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPME1
Gene ID: 51400
Uniprot ID: PPME1_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PPME1 (NP_057231.1).
Immunogen Sequence: MSALEKSMHLGRLPSRPPLP GSGGSQSGAKMRMGPGRKRD FSPVPWSQYFESMEDVEVEN ETGKDTFRVYKSGSEGPVLL LLHGGGHSALSWAVFTAAII SRVQCRIVALDLRSHGETKV KNPEDLSAETMAKDVGNVVE AMYGDLPPPIMLIGHSMGGA
Post Translational Modifications Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.
Function Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.
Protein Name Protein Phosphatase Methylesterase 1
Pme-1
Database Links Reactome: R-HSA-69273
Alternative Antibody Names Anti-Protein Phosphatase Methylesterase 1 antibody
Anti-Pme-1 antibody
Anti-PPME1 antibody
Anti-PME1 antibody
Anti-PP2593 antibody
Anti-PRO0750 antibody

Information sourced from Uniprot.org

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