• Western blot analysis of extracts of various cell lines, using PPP1R3B antibody (STJ117130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-PPP1R3B antibody (1-140) (STJ117130)

SKU:
STJ117130

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP1R3B (1-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R3B
Gene ID: 79660
Uniprot ID: PPR3B_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human PPP1R3B (NP_078883.2).
Immunogen Sequence: MMAVDIEYRYNCMAPSLRQE RFAFKISPKPSKPLRPCIQL SSKNEASGMVAPAVQEKKVK KRVSFADNQGLALTMVKVFS EFDDPLDMPFNITELLDNIV SLTTAESESFVLDFSQPSAD YLDFRNRLQADHVCLENCVL
Tissue Specificity Highly expressed in the liver and, at lower levels, in skeletal muscle, including in vastus lateralis, gastrocnemius and soleus (at protein level). Highest mRNA levels are observed in skeletal muscle, and only moderate levels in liver and heart. Weak expression in placenta and lung.
Function Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes.
Protein Name Protein Phosphatase 1 Regulatory Subunit 3b
Hepatic Glycogen-Targeting Protein Phosphatase 1 Regulatory Subunit Gl
Protein Phosphatase 1 Regulatory Subunit 4
Pp1 Subunit R4
Protein Phosphatase 1 Subunit Gl
Ptg
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 3b antibody
Anti-Hepatic Glycogen-Targeting Protein Phosphatase 1 Regulatory Subunit Gl antibody
Anti-Protein Phosphatase 1 Regulatory Subunit 4 antibody
Anti-Pp1 Subunit R4 antibody
Anti-Protein Phosphatase 1 Subunit Gl antibody
Anti-Ptg antibody
Anti-PPP1R3B antibody
Anti-PPP1R4 antibody

Information sourced from Uniprot.org

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