• Western blot analysis of various lysates using PPT1 Rabbit polyclonal antibody (STJ116969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PPT1 antibody (28-306) (STJ116969)

SKU:
STJ116969

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPT1 (28-306) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPT1
Gene ID: 5538
Uniprot ID: PPT1_HUMAN
Immunogen Region: 28-306
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-306 of human PPT1 (NP_000301.1).
Immunogen Sequence: DPPAPLPLVIWHGMGDSCCN PLSMGAIKKMVEKKIPGIYV LSLEIGKTLMEDVENSFFLN VNSQVTTVCQALAKDPKLQQ GYNAMGFSQGGQFLRAVAQR CPSPPMINLISVGGQHQGVF GLPRCPGESSHICDFIRKTL NAGAYSKVVQERLVQAEYWH DPIKEDVYRNHSIFLADINQ ERGINESYKKNLMALKKFVM VKFLNDSIVDPVDSEWFGFY RSGQAKETIPLQETSLYTQ
Post Translational Modifications Glycosylated.
Function Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
Protein Name Palmitoyl-Protein Thioesterase 1
Ppt-1
Palmitoyl-Protein Hydrolase 1
Database Links Reactome: R-HSA-75105
Cellular Localisation Lysosome
Secreted
Alternative Antibody Names Anti-Palmitoyl-Protein Thioesterase 1 antibody
Anti-Ppt-1 antibody
Anti-Palmitoyl-Protein Hydrolase 1 antibody
Anti-PPT1 antibody
Anti-CLN1 antibody
Anti-PPT antibody

Information sourced from Uniprot.org

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