• Western blot analysis of extracts of various cell lines, using PRDM11 antibody (STJ116078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRDM11 antibody (1-220) (STJ116078)

SKU:
STJ116078

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRDM11 (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDM11
Gene ID: 56981
Uniprot ID: PRD11_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRDM11 (NP_001243625.1).
Immunogen Sequence: MTENMKECLAQTNAAVGDMV TVVKTEVCSPLRDQEYGQPC SRRPDSSAMEVEPKKLKGKR DLIVPKSFQQVDFWFCESCQ EYFVDECPNHGPPVFVSDTP VPVGIPDRAALTIPQGMEVV KDTSGESDVRCVNEVIPKGH IFGPYEGQISTQDKSAGFFS WLIVDKNNRYKSIDGSDETK ANWMRYVVISREEREQNLLA FQHSERIYFRACRDIRPGEW
Tissue Specificity Highly expressed in lung, including bronchial epithelial cells and airway smooth muscle cells, as well as in peripheral blood mononuclear cells. In tonsils, expressed in B-cell types, including naive B-cells, centroblasts, centrocytes and memory B-cells (at protein level). In benign hyperplastic lymph nodes, expressed in germinal center cells in both the dark and light zones, as well as in scattered cells in the mantle zone and the interfollicular area (at protein level).
Function May be involved in transcription regulation.
Protein Name Pr Domain-Containing Protein 11
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Pr Domain-Containing Protein 11 antibody
Anti-PRDM11 antibody
Anti-PFM8 antibody

Information sourced from Uniprot.org

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