• Immunofluorescence analysis of NIH/3T3 cells using PRG2 Rabbit polyclonal antibody (STJ25110) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PRG2 Rabbit polyclonal antibody (STJ25110) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PRG2 in paraffin-embedded human placenta using PRG2 Rabbit polyclonal antibody (STJ25110) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse liver, using PRG2 Rabbit polyclonal antibody (STJ25110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRG2 antibody (100-200) (STJ25110)

SKU:
STJ25110

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRG2 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRG2
Gene ID: 5553
Uniprot ID: PRG2_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PRG2 (NP_002719.3).
Immunogen Sequence: GIPGCQTCRYLLVRSLQTFS QAWFTCRRCYRGNLVSIHNF NINYRIQCSVSALNQGQVWI GGRITGSGRCRRFQWVDGSR WNFAYWAAHQPWSRGGHCVA L
Tissue Specificity Detected in plasma and urine (at protein level). Detected in placenta (at protein level). High levels of the proform in placenta and pregnancy serum.in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.
Post Translational Modifications Nitrated.
Function Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
Protein Name Bone Marrow Proteoglycan
Bmpg
Proteoglycan 2 Cleaved Into - Eosinophil Granule Major Basic Protein
Embp
Mbp
Pregnancy-Associated Major Basic Protein
Database Links Reactome: R-HSA-6798695
Cellular Localisation Bone Marrow Proteoglycan: Secreted
The Proform Is Secreted
Eosinophil Granule Major Basic Protein: Cytoplasmic Vesicle
Secretory Vesicle
The Mature Protein Is Found In The Matrix Of The Eosinophil's Large Specific Granule (Crystalloid Core)
Alternative Antibody Names Anti-Bone Marrow Proteoglycan antibody
Anti-Bmpg antibody
Anti-Proteoglycan 2 Cleaved Into - Eosinophil Granule Major Basic Protein antibody
Anti-Embp antibody
Anti-Mbp antibody
Anti-Pregnancy-Associated Major Basic Protein antibody
Anti-PRG2 antibody
Anti-MBP antibody

Information sourced from Uniprot.org

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