Anti-PRKACA antibody

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Product name Anti-PRKACA antibody
Short Description Rabbit polyclonal against PRKACA
Description This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Applications IHC, WB
Dilution range WB 1:500 - 1:2000 IHC 1:50 - 1:200
Protein Name Camp-Dependent Protein Kinase Catalytic Subunit Alpha
Pka C-Alpha
Immunogen A synthetic peptide of human PRKACA
Storage Instruction Store at -20°C. Avoid freeze / thaw cycles.
Note For Research Use Only (RUO).
Validated Application
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Gene ID 5566
Gene Symbol PRKACA
Molecular Weight Calculated: 39kDa/40kDa. Observed: 41kDa
Alternative Names Anti-Camp-Dependent Protein Kinase Catalytic Subunit Alpha antibody
Anti-Pka C-Alpha antibody
Anti-PRKACA PKACA antibody
Function Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen. maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1. this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock.
Post-translational Modifications Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. ; Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity. ; Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR; this increases catalytic efficiency.
Cellular Localization Cytoplasm
Cell Membrane
Nucleus
Mitochondrion
Membrane
Lipid-Anchor
Translocates Into The Nucleus (Monomeric Catalytic Subunit)
The Inactive Holoenzyme Is Found In The Cytoplasm
Distributed Throughout The Cytoplasm In Meiotically Incompetent Oocytes
Associated To Mitochondrion As Meiotic Competence Is Acquired
Aggregates Around The Germinal Vesicles (Gv) At The Immature Gv Stage Oocytes (By Similarity)
Colocalizes With Hsf1 In Nuclear Stress Bodies (Nsbs) Upon Heat Shock (Pubmed:21085490)
Isoform 2: Cell Projection
Cilium
Flagellum
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Expressed In The Midpiece Region Of The Sperm Flagellum (Pubmed:10906071)
Colocalizes With Mroh2b And Tcp11 On The Acrosome And Tail Regions In Round Spermatids And Spermatozoa Regardless Of The Capacitation Status Of The Sperm (By Similarity)
Tissue Specificity Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
Swiss-Prot Key KAPCA_HUMAN
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