Anti-PRKACA Antibody

[STJ111027] Download PDF Print Data Sheet

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Product name Anti-PRKACA Antibody
Short Description Rabbit polyclonal against PRKACA
Description This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Applications IHC, WB
Dilution range WB 1:500 - 1:2000
IHC 1:50 - 1:200
Protein Name cAMP-dependent protein kinase catalytic subunit alpha
PKA C-alpha
Immunogen A synthetic peptide of human PRKACA
Storage Instruction Store at -20C. Avoid freeze / thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB IHC
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Gene ID 5566
Gene Symbol PRKACA
Molecular Weight 40.59 kDa
Database Links HGNC:9380
OMIM:601639
Reactome:R-HSA-111931
Alternative Names cAMP-dependent protein kinase catalytic subunit alpha
PKA C-alpha
Function Phosphorylates a large number of substrates in the cytoplasm and the nucleus, Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis, Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP, RORA is activated by phosphorylation, Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts, Involved in the regulation of platelets in response to thrombin and collagen
Post-translational Modifications Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively,
Cellular Localization Cytoplasm, Cell membrane, Nucleus,
Tissue Specificity Isoform 1 is ubiquitous, Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids
Swiss-Prot Key P17612
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