• Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PKA RII Alpha (PRKAR2A) /PKR2 antibody (STJ25123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts from normal (control) and PKA RII Alpha (PRKAR2A) /PKR2 knockout (KO) HeLa cells, using PKA RII Alpha (PRKAR2A) /PKR2 antibody (STJ25123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PRKAR2A antibody (1-404) (STJ25123)

SKU:
STJ25123

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRKAR2A (1-404) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAR2A
Gene ID: 5576
Uniprot ID: KAP2_HUMAN
Immunogen Region: 1-404
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PKA RII Alpha (PRKAR2A) /PKR2 (NP_004148.1).
Immunogen Sequence: MSHIQIPPGLTELLQGYTVE VLRQQPPDLVEFAVEYFTRL REARAPASVLPAATPRQSLG HPPPEPGPDRVADAKGDSES EEDEDLEVPVPSRFNRRVSV CAETYNPDEEEEDTDPRVIH PKTDEQRCRLQEACKDILLF KNLDQEQLSQVLDAMFERIV KADEHVIDQGDDGDNFYVIE RGTYDILVTKDNQTRSVGQY DNRGSFGELALMYNTPRAAT IVATSEGSLWGLDRVTFRR
Tissue Specificity Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Post Translational Modifications Phosphorylated by the activated catalytic chain.
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Protein Name Camp-Dependent Protein Kinase Type Ii-Alpha Regulatory Subunit
Database Links Reactome: R-HSA-163615
Reactome: R-HSA-164378
Reactome: R-HSA-180024
Reactome: R-HSA-381676
Reactome: R-HSA-432040
Reactome: R-HSA-442720
Reactome: R-HSA-5610787
Reactome: R-HSA-9010642
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Reactome: R-HSA-9664323
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Cell Membrane
Colocalizes With Pja2 In The Cytoplasm And The Cell Membrane
Alternative Antibody Names Anti-Camp-Dependent Protein Kinase Type Ii-Alpha Regulatory Subunit antibody
Anti-PRKAR2A antibody
Anti-PKR2 antibody
Anti-PRKAR2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance