Anti-PRKDC Antibody

[STJ25141] Download PDF Print Data Sheet

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Product name Anti-PRKDC Antibody
Short Description Rabbit polyclonal against PRKDC
Description This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.
Applications WB
Dilution range WB 1:500 - 1:2000
Protein Name DNA-dependent protein kinase catalytic subunit
DNA-PK catalytic subunit
DNA-PKcs
Immunogen A synthetic peptide of human PRKDC
Storage Instruction Store at -20C. Avoid freeze / thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Gene ID 5591
Gene Symbol PRKDC
Molecular Weight 469.089 kDa
Database Links HGNC:9413
OMIM:600899
Reactome:R-HSA-1834949
Alternative Names DNA-dependent protein kinase catalytic subunit
DNA-PK catalytic subunit
DNA-PKcs
Function Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage, Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination, Must be bound to DNA to express its catalytic properties, Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C), The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step, Required to protect and align broken ends of DNA, May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage, Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion, Also involved in modulation of transcription, Recognizes the substrate consensus sequence [ST]-Q, Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism, Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2, Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA, Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D, Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism, Interacts with CRY1 and CRY2
Post-translational Modifications Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647, Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C, Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair,
Cellular Localization Nucleus
Swiss-Prot Key P78527
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