• Western blot analysis of extracts of various cell lines, using PRMT7 antibody (STJ114052) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRMT7 antibody (513-692) (STJ114052)

SKU:
STJ114052

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRMT7 (513-692) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRMT7
Gene ID: 54496
Uniprot ID: ANM7_HUMAN
Immunogen Region: 513-692
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 513-692 of human PRMT7 (NP_061896.1).
Immunogen Sequence: QAASLHAVVVEFRDLWRIRS PCGDCEGFDVHIMDDMIKRA LDFRESREAEPHPLWEYPCR SLSEPWQILTFDFQQPVPLQ PLCAEGTVELRRPGQSHAAV LWMEYHLTPECTLSTGLLEP ADPEGGCCWNPHCKQAVYFF SPAPDPRALLGGPRTVSYAV EFHPDTGDIIMEFRHADTPD
Function Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3).such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro.the relevance of such results is however unclear in vivo.
Protein Name Protein Arginine N-Methyltransferase 7
Histone-Arginine N-Methyltransferase Prmt7
Myelin Basic Protein-Arginine N-Methyltransferase Prmt7
Database Links Reactome: R-HSA-3214858
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Alternative Antibody Names Anti-Protein Arginine N-Methyltransferase 7 antibody
Anti-Histone-Arginine N-Methyltransferase Prmt7 antibody
Anti-Myelin Basic Protein-Arginine N-Methyltransferase Prmt7 antibody
Anti-PRMT7 antibody
Anti-KIAA1933 antibody

Information sourced from Uniprot.org

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