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Anti-PSMD4 antibody (1-377) (STJ25189)

Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g PSMD4 antibody (STJ25189). Western blot was performed from the immunoprecipitate using PSMD4 antibody (STJ25189) at a dilution of 1:1000.
Immunofluorescence analysis of U2OS cells using PSMD4 Rabbit polyclonal antibody (STJ25189) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of H9C2 cells using PSMD4 Rabbit polyclonal antibody (STJ25189) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of lysates from HUVEC cells, using PSMD4 Rabbit polyclonal antibody (STJ25189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PSMD4 antibody (1-377) (STJ25189)

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STJ25189

£169.50 - £449.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IF/IP
Reactivity:	Human/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-PSMD4 (1-377) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IF/ICC 1:50-1:200 IP 1:500-1:1000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PSMD4
Gene ID:	5710
Uniprot ID:	PSMD4_HUMAN
Immunogen Region:	1-377
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human PSMD4 (NP_002801.1).
Immunogen Sequence:	MVLESTMVCVDNSEYMRNGD FLPTRLQAQQDAVNIVCHSK TRSNPENNVGLITLANDCEV LTTLTPDTGRILSKLHTVQP KGKITFCTGIRVAHLALKHR QGKNHKMRIIAFVGSPVEDN EKDLVKLAKRLKKEKVNVDI INFGEEEVNTEKLTAFVNTL NGKDGTGSHLVTVPPGPSLA DALISSPILAGEGGAMLGLG ASDFEFGVDPSADPELALAL RVSMEEQRQRQEEEARRAA

Function	Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
Protein Name	26s Proteasome Non-Atpase Regulatory Subunit 4 26s Proteasome Regulatory Subunit Rpn10 26s Proteasome Regulatory Subunit S5a Antisecretory Factor 1 Af Asf Multiubiquitin Chain-Binding Protein
Database Links	Reactome: R-HSA-1169091 Reactome: R-HSA-1234176 Reactome: R-HSA-1236974 Reactome: R-HSA-1236978 Reactome: R-HSA-174084 Reactome: R-HSA-174113 Reactome: R-HSA-174154 Reactome: R-HSA-174178 Reactome: R-HSA-174184 Reactome: R-HSA-180534 Reactome: R-HSA-180585 Reactome: R-HSA-187577 Reactome: R-HSA-195253 Reactome: R-HSA-202424 Reactome: R-HSA-211733 Reactome: R-HSA-2467813 Reactome: R-HSA-2871837 Reactome: R-HSA-349425 Reactome: R-HSA-350562 Reactome: R-HSA-382556 Reactome: R-HSA-450408 Reactome: R-HSA-4608870 Reactome: R-HSA-4641257 Reactome: R-HSA-4641258 Reactome: R-HSA-5358346 Reactome: R-HSA-5362768 Reactome: R-HSA-5607761 Reactome: R-HSA-5607764 Reactome: R-HSA-5610780 Reactome: R-HSA-5610783 Reactome: R-HSA-5610785 Reactome: R-HSA-5632684 Reactome: R-HSA-5658442 Reactome: R-HSA-5668541 Reactome: R-HSA-5676590 Reactome: R-HSA-5678895 Reactome: R-HSA-5687128 Reactome: R-HSA-5689603 Reactome: R-HSA-5689880 Reactome: R-HSA-68867 Reactome: R-HSA-68949 Reactome: R-HSA-69017 Reactome: R-HSA-69481 Reactome: R-HSA-69601 Reactome: R-HSA-75815 Reactome: R-HSA-8852276 Reactome: R-HSA-8854050 Reactome: R-HSA-8939236 Reactome: R-HSA-8939902 Reactome: R-HSA-8941858 Reactome: R-HSA-8948751 Reactome: R-HSA-8951664 Reactome: R-HSA-9010553 Reactome: R-HSA-9020702 Reactome: R-HSA-9604323 Reactome: R-HSA-9755511 Reactome: R-HSA-9762114 Reactome: R-HSA-9824272 Reactome: R-HSA-983168
Alternative Antibody Names	Anti-26s Proteasome Non-Atpase Regulatory Subunit 4 antibody Anti-26s Proteasome Regulatory Subunit Rpn10 antibody Anti-26s Proteasome Regulatory Subunit S5a antibody Anti-Antisecretory Factor 1 antibody Anti-Af antibody Anti-Asf antibody Anti-Multiubiquitin Chain-Binding Protein antibody Anti-PSMD4 antibody Anti-MCB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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