• Western blot analysis of extracts of various cell lines, using PTN antibody (STJ115989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PTN antibody (C-Term) (STJ115989)

SKU:
STJ115989

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTN (C-Term) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTN
Gene ID: 5764
Uniprot ID: PTN_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PTN (NP_002816.1).
Immunogen Sequence: KYQFQAWGECDLNTALKTRT GSLKRALHNAECQKTVTISK PCGKLTKPKPQAESKKKKKE GKKQEKMLD
Tissue Specificity Osteoblast and brain.
Post Translational Modifications Phosphorylated by NEK6.
Function Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK, CTNNB1 or AFAP1L2 in order to activate the PI3K-AKT pathway. Through PTPRZ1 binding controls oligodendrocyte precursor cell differentiation by enhancing the phosphorylation of AFAP1L2 in order to activate the PI3K-AKT pathway. Forms a complex with PTPRZ1 and integrin alpha-V/beta-3 (ITGAV:ITGB3) that stimulates endothelial cell migration through SRC dephosphorylation and activation that consequently leads to ITGB3 'Tyr-773' phosphorylation. In adult hippocampus promotes dendritic arborization, spine development, and functional integration and connectivity of newborn granule neurons through ALK by activating AKT signaling pathway. Binds GPC2 and chondroitin sulfate proteoglycans (CSPGs) at the neuron surface, leading to abrogation of binding between PTPRS and CSPGs and neurite outgrowth promotion. Binds SDC3 and mediates bone formation by recruiting and attaching osteoblasts/osteoblast precursors to the sites for new bone deposition. Binds ALK and promotes cell survival and cell proliferation through MAPK pathway activation. Inhibits proliferation and enhances differentiation of neural stem cells by inhibiting FGF2-induced fibroblast growth factor receptor signaling pathway. Mediates regulatory mechanisms in normal hemostasis and in hematopoietic regeneration and in maintaining the balance of myeloid and lymphoid regeneration. In addition may play a role in the female reproductive system, auditory response and the progesterone-induced decidualization pathway.
Protein Name Pleiotrophin
Ptn
Heparin-Binding Brain Mitogen
Hbbm
Heparin-Binding Growth Factor 8
Hbgf-8
Heparin-Binding Growth-Associated Molecule
Hb-Gam
Heparin-Binding Neurite Outgrowth-Promoting Factor
Hbnf
Heparin-Binding Neurite Outgrowth-Promoting Factor 1
Hbnf-1
Osteoblast-Specific Factor 1
Osf-1
Database Links Reactome: R-HSA-201556
Cellular Localisation Secreted
Alternative Antibody Names Anti-Pleiotrophin antibody
Anti-Ptn antibody
Anti-Heparin-Binding Brain Mitogen antibody
Anti-Hbbm antibody
Anti-Heparin-Binding Growth Factor 8 antibody
Anti-Hbgf-8 antibody
Anti-Heparin-Binding Growth-Associated Molecule antibody
Anti-Hb-Gam antibody
Anti-Heparin-Binding Neurite Outgrowth-Promoting Factor antibody
Anti-Hbnf antibody
Anti-Heparin-Binding Neurite Outgrowth-Promoting Factor 1 antibody
Anti-Hbnf-1 antibody
Anti-Osteoblast-Specific Factor 1 antibody
Anti-Osf-1 antibody
Anti-PTN antibody
Anti-HBNF1 antibody
Anti-NEGF1 antibody

Information sourced from Uniprot.org

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