• Western blot analysis of extracts of various cell lines, using RAB13 antibody (STJ112586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using RAB13 rabbit polyclonal antibody (STJ112586) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RAB13 antibody (1-203) (STJ112586)

SKU:
STJ112586

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB13 (1-203) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB13
Gene ID: 5872
Uniprot ID: RAB13_HUMAN
Immunogen Region: 1-203
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human RAB13 (NP_002861.1).
Immunogen Sequence: MAKAYDHLFKLLLIGDSGVG KTCLIIRFAEDNFNNTYIST IGIDFKIRTVDIEGKKIKLQ VWDTAGQERFKTITTAYYRG AMGIILVYDITDEKSFENIQ NWMKSIKENASAGVERLLLG NKCDMEAKRKVQKEQADKLA REHGIRFFETSAKSSMNVDE AFSSLARDILLKSGGRRSGN GNKPPSTDLKTCDKKNTNKC SLG
Tissue Specificity Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.
Post Translational Modifications (Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.
Protein Name Ras-Related Protein Rab-13
Cell Growth-Inhibiting Gene 4 Protein
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cytoplasmic Vesicle Membrane
Cell Junction
Tight Junction
Golgi Apparatus
Trans-Golgi Network Membrane
Recycling Endosome Membrane
Cell Projection
Lamellipodium
Tight Junctions Or Associated With Vesicles Scattered Throughout The Cytoplasm In Cells Lacking Tight Junctions
Relocalizes To The Leading Edge Of Lamellipodia In Migrating Endothelial Cells
Alternative Antibody Names Anti-Ras-Related Protein Rab-13 antibody
Anti-Cell Growth-Inhibiting Gene 4 Protein antibody
Anti-RAB13 antibody
Anti-GIG4 antibody

Information sourced from Uniprot.org

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