• Western blot analysis of extracts of various cell lines, using RAB18 antibody (STJ25256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RAB18 antibody (1-206) (STJ25256)

SKU:
STJ25256

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB18 (1-206) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB18
Gene ID: 22931
Uniprot ID: RAB18_HUMAN
Immunogen Region: 1-206
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human RAB18 (NP_067075.1).
Immunogen Sequence: MDEDVLTTLKILIIGESGVG KSSLLLRFTDDTFDPELAAT IGVDFKVKTISVDGNKAKLA IWDTAGQERFRTLTPSYYRG AQGVILVYDVTRRDTFVKLD NWLNELETYCTRNDIVNMLV GNKIDKENREVDRNEGLKFA RKHSMLFIEASAKTCDGVQC AFEELVEKIIQTPGLWESEN QNKGVKLSHREEGQGGGACG GYCSVL
Tissue Specificity Ubiquitous.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the localization of ZFYVE1 to lipid droplets and for its function in mediating the formation of endoplasmic reticulum-lipid droplets (ER-LD) contacts. Also required for maintaining endoplasmic reticulum structure. Plays a role in apical endocytosis/recycling. Plays a key role in eye and brain development and neurodegeneration.
Protein Name Ras-Related Protein Rab-18
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-6811436
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Apical Cell Membrane
Lipid Droplet
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Ras-Related Protein Rab-18 antibody
Anti-RAB18 antibody

Information sourced from Uniprot.org

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