• Western blot analysis of extracts of various cell lines, using RAB3A antibody (STJ27332) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of mouse brain using RAB3A antibody (STJ27332) at dilution of 1:50. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat brain using RAB3A antibody (STJ27332) at dilution of 1:50. Blue: DAPI for nuclear staining.

Anti-RAB3A antibody (100-200) (STJ27332)

SKU:
STJ27332

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB3A (100-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB3A
Gene ID: 5864
Uniprot ID: RAB3A_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RAB3A (NP_002857.1).
Immunogen Sequence: LMYDITNEESFNAVQDWSTQ IKTYSWDNAQVLLVGNKCDM EDERVVSSERGRQLADHLGF EFFEASAKDNINVKQTFERL VDVICEKMSESLDTADPAVT G
Tissue Specificity Specifically expressed in brain.
Post Translational Modifications Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Function Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. Interacts with MADD (via uDENN domain).the GTP-bound form is preferred for interaction.
Protein Name Ras-Related Protein Rab-3a
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Reactome: R-HSA-888590
Reactome: R-HSA-9662360
Cellular Localisation Cytoplasm
Cytosol
Lysosome
Cytoplasmic Vesicle
Secretory Vesicle
Cell Projection
Axon
Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Presynapse
Postsynapse
Cycles Between A Vesicle-Associated Gtp-Bound Form And A Cytosolic Gdp-Bound Form
Alternative Antibody Names Anti-Ras-Related Protein Rab-3a antibody
Anti-RAB3A antibody

Information sourced from Uniprot.org

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