• Western blot analysis of extracts of U-87MG cells, using RAB8B rabbit polyclonal antibody (STJ26440) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RAB8B antibody (1-207) (STJ26440)

SKU:
STJ26440

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB8B (1-207) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB8B
Gene ID: 51762
Uniprot ID: RAB8B_HUMAN
Immunogen Region: 1-207
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human RAB8B (NP_057614.1).
Immunogen Sequence: MAKTYDYLFKLLLIGDSGVG KTCLLFRFSEDAFNTTFIST IGIDFKIRTIELDGKKIKLQ IWDTAGQERFRTITTAYYRG AMGIMLVYDITNEKSFDNIK NWIRNIEEHASSDVERMILG NKCDMNDKRQVSKERGEKLA IDYGIKFLETSAKSSANVEE AFFTLARDIMTKLNRKMNDS NSAGAGGPVKITENRSKKTS FFRCSLL
Post Translational Modifications Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may be involved in polarized vesicular trafficking and neurotransmitter release. May participate in cell junction dynamics in Sertoli cells. May participate in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route.
Protein Name Ras-Related Protein Rab-8b
Database Links Reactome: R-HSA-8854214
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cytoplasmic Vesicle
Phagosome
Phagosome Membrane
Endosome Membrane
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Colocalized With Mical1
Graf1/Arhgap26 And Graf2/Arhgap10 On Endosomal Tubules
Alternative Antibody Names Anti-Ras-Related Protein Rab-8b antibody
Anti-RAB8B antibody

Information sourced from Uniprot.org

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