• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RAN Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from LOVO cells, using RAN Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Ran Polyclonal Antibody diluted at 1:2000

Anti-RAN antibody (167-216 aa) (STJ95363)

SKU:
STJ95363

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTP-binding nuclear protein Ran (167-216 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAN
Gene ID: 5901
Uniprot ID: RAN_HUMAN
Immunogen Region: 167-216 aa
Specificity: Ran Polyclonal Antibody detects endogenous levels of Ran protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RAN at the amino acid range 167-216
Post Translational Modifications Acetylation by KAT5 at Lys-134 is increased during mitosis, impairs RANGRF binding and enhances RCC1 binding. Acetylation at Lys-37 enhances the association with nuclear export components. Deacetylation of Lys-37 by SIRT7 regulates the nuclear export of NF-kappa-B subunit RELA/p65.
Function GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Protein Name Gtp-Binding Nuclear Protein Ran
Androgen Receptor-Associated Protein 24
Gtpase Ran
Ras-Like Protein Tc4
Ras-Related Nuclear Protein
Database Links Reactome: R-HSA-165054
Reactome: R-HSA-1655829
Reactome: R-HSA-168333
Reactome: R-HSA-180746
Reactome: R-HSA-203927
Reactome: R-HSA-5578749
Reactome: R-HSA-6784531
Reactome: R-HSA-9615933
Cellular Localisation Nucleus
Nucleus Envelope
Cytoplasm
Cytosol
Melanosome
Predominantly Nuclear During Interphase
Becomes Dispersed Throughout The Cytoplasm During Mitosis
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Gtp-Binding Nuclear Protein Ran antibody
Anti-Androgen Receptor-Associated Protein 24 antibody
Anti-Gtpase Ran antibody
Anti-Ras-Like Protein Tc4 antibody
Anti-Ras-Related Nuclear Protein antibody
Anti-RAN antibody
Anti-ARA24 antibody
Anti-OK antibody
Anti-SW-cl.81 antibody

Information sourced from Uniprot.org

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