• Western blot analysis of extracts of various cell lines, using RDH5 antibody (STJ110358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of rat retina using RDH5 Polyclonal Antibody (STJ110358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse retina using RDH5 Polyclonal Antibody (STJ110358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RDH5 antibody (24-318) (STJ110358)

SKU:
STJ110358

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RDH5 (24-318) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RDH5
Gene ID: 5959
Uniprot ID: RDH5_HUMAN
Immunogen Region: 24-318
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-318 of human RDH5 (NP_002896.2).
Immunogen Sequence: LPASNAFVFITGCDSGFGRL LALQLDQRGFRVLASCLTPS GAEDLQRVASSRLHTTLLDI TDPQSVQQAAKWVEMHVKEA GLFGLVNNAGVAGIIGPTPW LTRDDFQRVLNVNTMGPIGV TLALLPLLQQARGRVINITS VLGRLAANGGGYCVSKFGLE AFSDSLRRDVAHFGIRVSIV EPGFFRTPVTNLESLEKTLQ ACWARLPPATQAHYGGAFLT KYLKMQQRIMNLICDPDLT
Tissue Specificity Widely expressed. In the eye, abundant in the retinal pigment epithelium.
Function Catalyzes the oxidation of cis-isomers of retinol, including 11-cis-, 9-cis-, and 13-cis-retinol in an NAD-dependent manner. Has no activity towards all-trans retinal. Plays a significant role in 11-cis retinol oxidation in the retinal pigment epithelium cells (RPE). Also recognizes steroids (androsterone, androstanediol) as its substrates.
Protein Name Retinol Dehydrogenase 5
11-Cis Retinol Dehydrogenase
11-Cis Rdh
11-Cis Rodh
9-Cis Retinol Dehydrogenase
9crdh
Short Chain Dehydrogenase/Reductase Family 9c Member 5
Database Links Reactome: R-HSA-2453864
Reactome: R-HSA-2453902
Reactome: R-HSA-5365859
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Lumenal Side
Alternative Antibody Names Anti-Retinol Dehydrogenase 5 antibody
Anti-11-Cis Retinol Dehydrogenase antibody
Anti-11-Cis Rdh antibody
Anti-11-Cis Rodh antibody
Anti-9-Cis Retinol Dehydrogenase antibody
Anti-9crdh antibody
Anti-Short Chain Dehydrogenase/Reductase Family 9c Member 5 antibody
Anti-RDH5 antibody
Anti-HSD17B9 antibody
Anti-RDH1 antibody
Anti-SDR9C5 antibody

Information sourced from Uniprot.org

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