Anti-RFFL antibody (1-190) (STJ115564)

SKU:
STJ115564

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RFFL (1-190) is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RFFL
Gene ID: 117584
Uniprot ID: RFFL_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RFFL (NP_001017368.1).
Immunogen Sequence: MWATCCNWFCLDGQPEEVPP PQGARMQAYSNPGYSSFPSP TGLEPSCKSCGAHFANTARK QTCLDCKKNFCMTCSSQVGN GPRLCLLCQRFRATAFQREE LMKMKVKDLRDYLSLHDIST EMCREKEELVLLVLGQQPVI SQEDRTRASTLSPDFPEQQA FLTQPHSSMVPPTSPNLPSS SAQATSVPPA
Tissue Specificity Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Post Translational Modifications Autoubiquitinated. Palmitoylated. Undergoes caspase-mediated cleavage upon death-receptor activation, by TNFSF10 for instance. May be mediated by the caspases CASP8 and CASP10 in a negative feedback loop.
Function E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.
Protein Name E3 Ubiquitin-Protein Ligase Rififylin
Caspase Regulator Carp2
Caspases-8 And -10-Associated Ring Finger Protein 2
Carp-2
Fyve-Ring Finger Protein Sakura
Fring
Ring Finger And Fyve-Like Domain-Containing Protein 1
Ring Finger Protein 189
Ring Finger Protein 34-Like
Ring-Type E3 Ubiquitin Transferase Rififylin
Database Links Reactome: R-HSA-6804757
Cellular Localisation Cytoplasm
Cytosol
Cell Membrane
Peripheral Membrane Protein
Recycling Endosome Membrane
The Fyve-Type Zinc Finger May Mediate Phosphatidylinositol Phosphate-Binding And Control Subcellular Localization
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rififylin antibody
Anti-Caspase Regulator Carp2 antibody
Anti-Caspases-8 And -10-Associated Ring Finger Protein 2 antibody
Anti-Carp-2 antibody
Anti-Fyve-Ring Finger Protein Sakura antibody
Anti-Fring antibody
Anti-Ring Finger And Fyve-Like Domain-Containing Protein 1 antibody
Anti-Ring Finger Protein 189 antibody
Anti-Ring Finger Protein 34-Like antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rififylin antibody
Anti-RFFL antibody
Anti-RNF189 antibody
Anti-RNF34L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance