• Immunofluorescence analysis of paraffin-embedded rat spleen using RNASE6 Rabbit polyclonal antibody (STJ117507) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RNASE6 in paraffin-embedded human appendix using RNASE6 Rabbit polyclonal antibody (STJ117507) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RNASE6 in paraffin-embedded human lung cancer using RNASE6 Rabbit polyclonal antibody (STJ117507) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse kidney, using RNASE6 Rabbit polyclonal antibody (STJ117507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNASE6 antibody (1-150) (STJ117507)

SKU:
STJ117507

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNASE6 (1-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNASE6
Gene ID: 6039
Uniprot ID: RNAS6_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human RNASE6 (NP_005606.1).
Immunogen Sequence: MVLCFPLLLLLLVLWGPVCP LHAWPKRLTKAHWFEIQHIQ PSPLQCNRAMSGINNYTQHC KHQNTFLHDSFQNVAAVCDL LSIVCKNRRHNCHQSSKPVN MTDCRLTSGKYPQCRYSAAA QYKFFIVACDPPQKSDPPYK LVPVHLDSIL
Tissue Specificity Highly expressed in spleen (at protein level). Has little or no expression in healthy kidneys (at protein level). Detected in interstitial leukocytes in infected kidneys (at protein level). Expressed in ureter where it localizes to urothelial and submucosal leukocytes (at protein level). Strong expression in lung and thymus, and lower expression in heart, placenta, pancreas, liver, brain and skeletal muscle. Also expressed in monocytes and neutrophils.
Function Ribonuclease which shows a preference for the pyrimidines uridine and cytosine. Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli. Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria. Probably contributes to urinary tract sterility. Bactericidal activity is independent of RNase activity.
Protein Name Ribonuclease K6
Rnase K6
Database Links Reactome: R-HSA-6803157
Cellular Localisation Secreted
Lysosome
Cytoplasmic Granule
Alternative Antibody Names Anti-Ribonuclease K6 antibody
Anti-Rnase K6 antibody
Anti-RNASE6 antibody
Anti-RNS6 antibody

Information sourced from Uniprot.org

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