• Western blot analysis of extracts of various cell lines, using RNF31 antibody (STJ116074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of human breast using RNF31 Polyclonal Antibody (STJ116074) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RNF31 antibody (500-700) (STJ116074)

SKU:
STJ116074

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF31 (500-700) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF31
Gene ID: 55072
Uniprot ID: RNF31_HUMAN
Immunogen Region: 500-700
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human RNF31 (NP_060469.4).
Immunogen Sequence: AAGACPEEIFSALQYSGTEV PLQWLRSELPYVLEMVAELA GQQDPGLGAFSCQEARRAWL DRHGNLDEAVEECVRTRRRK VQELQSLGFGPEEGSLQALF QHGGDVSRALTELQRQRLEP FRQRLWDSGPEPTPSWDGPD KQSLVRRLLAVYALPSWGRA ELALSLLQETPRNYELGDVV EAVRHSQDRAFLRRLLAQEC A
Tissue Specificity Expressed in both normal and transformed breast epithelial cell lines.
Post Translational Modifications Autoubiquitinated. Interaction with OTULIN is required to suppress formation of 'Met-1'-linked polyubiquitin chains and prevent subsequent inactivation of the LUBAC complex. Cleaved by caspase during apoptosis. (Microbial infection) Ubiquitinated by S.flexneri E3 ubiquitin-protein ligases IpaH1.4 and IpaH2.5, leading to its degradation by the proteasome, thereby preventing formation of the bacterial ubiquitin coat and activation of innate immunity.
Function E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin. Recruited to the surface of bacteria by RNF213, which initiates the bacterial ubiquitin coat. The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. RNF31 is required for linear ubiquitination of BCL10, thereby promoting TCR-induced NF-kappa-B activation. Binds polyubiquitin of different linkage types.
Protein Name E3 Ubiquitin-Protein Ligase Rnf31
Hoil-1-Interacting Protein
Hoip
Ring Finger Protein 31
Ring-Type E3 Ubiquitin Transferase Rnf31
Zinc In-Between-Ring-Finger Ubiquitin-Associated Domain Protein
Database Links Reactome: R-HSA-5357786
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf31 antibody
Anti-Hoil-1-Interacting Protein antibody
Anti-Hoip antibody
Anti-Ring Finger Protein 31 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rnf31 antibody
Anti-Zinc In-Between-Ring-Finger Ubiquitin-Associated Domain Protein antibody
Anti-RNF31 antibody
Anti-ZIBRA antibody

Information sourced from Uniprot.org

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