Anti-ROBO1/DUTT1 antibody

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Product name Anti-ROBO1/DUTT1 antibody
Short Description Unconjugated goat polyclonal antibody against ROBO1/DUTT1.
Applications ELISA, FC, IF, IHC, WB
Dilution range ELISA-antibody detection limit dilution 1:8000.
WB-An anonymous source observed an approx 250kDa band in Human Endothelial cell lysates at 1ug/ml antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.
IHC-Paraffin embedded Human Liver. Recommended concentration: 2µg/ml.
IF-Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10µg/ml.
Specificity Not expected to cross-react with ROBO2 or ROBO3.
Protein Name Roundabout Homolog 1
Deleted In U Twenty Twenty
H-Robo-1
Immunogen Region Internal
Storage Instruction Store at-20°C and avoid freeze-thaw cycles.
Note FOR RESEARCH USE ONLY (RUO).
Host Goat
Clonality Polyclonal
Reactivity Cow, Dog, Human, Mouse, Rat
Conjugation Unconjugated
Concentration 0.5 mg/ml
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Gene ID 6091
Gene Symbol ROBO1
Database Links HGNC:10249
OMIM:602430
Reactome:R-HSA-373752
Alternative Names Anti-Roundabout Homolog 1 antibody
Anti-Deleted In U Twenty Twenty antibody
Anti-H-Robo-1 antibody
Anti-ROBO1 DUTT1 antibody
Function Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B.inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.
Post-translational Modifications Ubiquitinated. May be deubiquitinated by USP33.
Cellular Localization Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Axon
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Single-Pass Membrane Protein
Detected At Growth Cones In Thalamus Neurons
Prrg4 Prevents Cell Surface Location And Both Colocalize In The Endoplasmic Reticulum/Golgi Adjacent To The Cell Nucleus
Tissue Specificity Widely expressed, with exception of kidney.
Swiss-Prot Key ROBO1_HUMAN
Sequence GDVDLSNKINEMK
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