• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from K562 cells, using RFA2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-RPA2 antibody (10-59 aa) (STJ95535)

SKU:
STJ95535

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Replication protein A 32 kDa subunit (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPA2
Gene ID: 6118
Uniprot ID: RFA2_HUMAN
Immunogen Region: 10-59 aa
Specificity: RPA32 Polyclonal Antibody detects endogenous levels of RPA32 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RFA2 at the amino acid range 10-59
Function As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Protein Name Replication Protein A 32 Kda Subunit
Rp-A P32
Replication Factor A Protein 2
Rf-A Protein 2
Replication Protein A 34 Kda Subunit
Rp-A P34
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174437
Reactome: R-HSA-176187
Reactome: R-HSA-3371453
Reactome: R-HSA-3371511
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-5696395
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6783310
Reactome: R-HSA-6804756
Reactome: R-HSA-68962
Reactome: R-HSA-69166
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Pml Body
Redistributes To Discrete Nuclear Foci Upon Dna Damage In An Atr-Dependent Manner
Alternative Antibody Names Anti-Replication Protein A 32 Kda Subunit antibody
Anti-Rp-A P32 antibody
Anti-Replication Factor A Protein 2 antibody
Anti-Rf-A Protein 2 antibody
Anti-Replication Protein A 34 Kda Subunit antibody
Anti-Rp-A P34 antibody
Anti-RPA2 antibody
Anti-REPA2 antibody
Anti-RPA32 antibody
Anti-RPA34 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance