• Western blot analysis of extracts of various cell lines, using RSPO3 antibody (STJ110687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RSPO3 antibody (1-100) (STJ110687)

SKU:
STJ110687

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RSPO3 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RSPO3
Gene ID: 84870
Uniprot ID: RSPO3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RSPO3 (NP_116173.2).
Immunogen Sequence: MHLRLISWLFIILNFMEYIG SQNASRGRRQRRMHPNVSQG CQGGCATCSDYNGCLSCKPR LFFALERIGMKQIGVCLSSC PSGYYGTRYPDINKCTKCKA
Tissue Specificity Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node. Highly expressed in endothelial cells.
Function Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.
Protein Name R-Spondin-3
Protein With Tsp Type-1 Repeat
Hpwtsr
Roof Plate-Specific Spondin-3
Hrspo3
Thrombospondin Type-1 Domain-Containing Protein 2
Database Links Reactome: R-HSA-4641263
Reactome: R-HSA-8939256
Cellular Localisation Secreted
Alternative Antibody Names Anti-R-Spondin-3 antibody
Anti-Protein With Tsp Type-1 Repeat antibody
Anti-Hpwtsr antibody
Anti-Roof Plate-Specific Spondin-3 antibody
Anti-Hrspo3 antibody
Anti-Thrombospondin Type-1 Domain-Containing Protein 2 antibody
Anti-RSPO3 antibody
Anti-PWTSR antibody
Anti-THSD2 antibody

Information sourced from Uniprot.org

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