• Western blot analysis of extracts of various cell lines, using RUNX2 antibody (STJ25424) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of mouse testis, using RUNX2 antibody (STJ25424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded rat spleen using RUNX2 rabbit polyclonal antibody (STJ25424) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse salivary gland using RUNX2 rabbit polyclonal antibody (STJ25424) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of PC-3 cells using RUNX2 rabbit polyclonal antibody (STJ25424) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-RUNX2 antibody (242-521) (STJ25424)

SKU:
STJ25424

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RUNX2 (242-521) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RUNX2
Gene ID: 860
Uniprot ID: RUNX2_HUMAN
Immunogen Region: 242-521
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 242-521 of human RUNX2 (NP_001019801.3).
Immunogen Sequence: FSDRLSDLGRIPHPSMRVGV PPQNPRPSLNSAPSPFNPQG QSQITDPRQAQSSPPWSYDQ SYPSYLSQMTSPSIHSTTPL SSTRGTGLPAITDVPRRISD DDTATSDFCLWPSTLSKKSQ AGASELGPFSDPRQFPSISS LTESRFSNPRMHYPATFTYT PPVTSGMSLGMSATTHYHTY LPPPYPGSSQSQSGPFQTSS TPYLYYGTSSGSYQFPMVPG GDRSPSRMLPPCTTTSNGS
Tissue Specificity Specifically expressed in osteoblasts.
Post Translational Modifications Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation. Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340.
Function Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation.
Protein Name Runt-Related Transcription Factor 2
Acute Myeloid Leukemia 3 Protein
Core-Binding Factor Subunit Alpha-1
Cbf-Alpha-1
Oncogene Aml-3
Osteoblast-Specific Transcription Factor 2
Osf-2
Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit
Pea2-Alpha A
Pebp2-Alpha A
Sl3-3 Enhancer Factor 1 Alpha A Subunit
Sl3/Akv Core-Binding Factor Alpha A Subunit
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-8878166
Reactome: R-HSA-8939246
Reactome: R-HSA-8939902
Reactome: R-HSA-8940973
Reactome: R-HSA-8941284
Reactome: R-HSA-8941326
Reactome: R-HSA-8941332
Reactome: R-HSA-8941333
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Runt-Related Transcription Factor 2 antibody
Anti-Acute Myeloid Leukemia 3 Protein antibody
Anti-Core-Binding Factor Subunit Alpha-1 antibody
Anti-Cbf-Alpha-1 antibody
Anti-Oncogene Aml-3 antibody
Anti-Osteoblast-Specific Transcription Factor 2 antibody
Anti-Osf-2 antibody
Anti-Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit antibody
Anti-Pea2-Alpha A antibody
Anti-Pebp2-Alpha A antibody
Anti-Sl3-3 Enhancer Factor 1 Alpha A Subunit antibody
Anti-Sl3/Akv Core-Binding Factor Alpha A Subunit antibody
Anti-RUNX2 antibody
Anti-AML3 antibody
Anti-CBFA1 antibody
Anti-OSF2 antibody
Anti-PEBP2A antibody

Information sourced from Uniprot.org

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