• Immunohistochemistry analysis of SAG in paraffin-embedded mouse retina using SAG Rabbit polyclonal antibody (STJ115012) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat eye, using SAG Rabbit polyclonal antibody (STJ115012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from Mouse eye, using SAG Rabbit polyclonal antibody (STJ115012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SAG antibody (1-405) (STJ115012)

SKU:
STJ115012

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SAG (1-405) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SAG
Gene ID: 6295
Uniprot ID: ARRS_HUMAN
Immunogen Region: 1-405
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human SAG (NP_000532.2).
Immunogen Sequence: MAASGKTSKSEPNHVIFKKI SRDKSVTIYLGNRDYIDHVS QVQPVDGVVLVDPDLVKGKK VYVTLTCAFRYGQEDIDVIG LTFRRDLYFSRVQVYPPVGA ASTPTKLQESLLKKLGSNTY PFLLTFPDYLPCSVMLQPAP QDSGKSCGVDFEVKAFATDS TDAEEDKIPKKSSVRLLIRK VQHAPLEMGPQPRAEAAWQF FMSDKPLHLAVSLNKEIYFH GEPIPVTVTVTNNTEKTVK
Tissue Specificity Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level).
Function Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO. May play a role in preventing light-dependent degeneration of retinal photoreceptor cells.
Protein Name S-Arrestin
48 Kda Protein
Retinal S-Antigen
S-Ag
Rod Photoreceptor Arrestin
Database Links Reactome: R-HSA-2485179
Reactome: R-HSA-2514859
Cellular Localisation Cell Projection
Cilium
Photoreceptor Outer Segment
Membrane
Peripheral Membrane Protein
Highly Expressed In Photoreceptor Outer Segments In Light-Exposed Retina
Evenly Distributed Throughout Rod Photoreceptor Cells In Dark-Adapted Retina
Predominantly Dectected At The Proximal Region Of Photoreceptor Outer Segments
Near Disk Membranes
Alternative Antibody Names Anti-S-Arrestin antibody
Anti-48 Kda Protein antibody
Anti-Retinal S-Antigen antibody
Anti-S-Ag antibody
Anti-Rod Photoreceptor Arrestin antibody
Anti-SAG antibody

Information sourced from Uniprot.org

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