• Western blot analysis of various lysates using SEC22B Rabbit polyclonal antibody (STJ117553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SEC22B antibody (85-195) (STJ117553)

SKU:
STJ117553

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEC22B (85-195) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEC22B
Gene ID: 9554
Uniprot ID: SC22B_HUMAN
Immunogen Region: 85-195
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 85-195 of human SEC22B (NP_004883.3).
Immunogen Sequence: FAYLEDLHSEFDEQHGKKVP TVSRPYSFIEFDTFIQKTKK LYIDSRARRNLGSINTELQD VQRIMVANIEEVLQRGEALS ALDSKANNLSSLSKKYRQDA KYLNMRSTYAK
Function SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
Protein Name Vesicle-Trafficking Protein Sec22b
Er-Golgi Snare Of 24 Kda
Ers-24
Ers24
Sec22 Vesicle-Trafficking Protein Homolog B
Sec22 Vesicle-Trafficking Protein-Like 1
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-6811434
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Golgi Apparatus
Cis-Golgi Network Membrane
Trans-Golgi Network Membrane
Melanosome
Concentrated Most In The Intermediate Compartment/Cis-Golgi Network And The Cis-Golgi Cisternae 1 And 2
Greatly Reduced In Concentration At The Trans End Of The Golgi Apparatus
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Vesicle-Trafficking Protein Sec22b antibody
Anti-Er-Golgi Snare Of 24 Kda antibody
Anti-Ers-24 antibody
Anti-Ers24 antibody
Anti-Sec22 Vesicle-Trafficking Protein Homolog B antibody
Anti-Sec22 Vesicle-Trafficking Protein-Like 1 antibody
Anti-SEC22B antibody
Anti-SEC22L1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance