• Western blot analysis of extracts of various cell lines, using SIAE antibody (STJ115183) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SIAE antibody (270-450) (STJ115183)

SKU:
STJ115183

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIAE (270-450) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIAE
Gene ID: 54414
Uniprot ID: SIAE_HUMAN
Immunogen Region: 270-450
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-450 of human SIAE (NP_733746.1).
Immunogen Sequence: LKGVVWYQGESNINYNTDLY NCTFPALIEDWRETFHRGSQ GQTERFFPFGLVQLSSDLSK KSSDDGFPQIRWHQTADFGY VPNPKMPNTFMAVAMDLCDR DSPFGSIHPRDKQTVAYRLH LGARALAYGEKNLTFEGPLP EKIELLAHKGLLNLTYYQQI QVQKKDNKIFEISCCSDHRC K
Tissue Specificity Widely expressed with high expression in the testis, prostate, and colon.
Function Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.
Protein Name Sialate O-Acetylesterase
H-Lse
Sialic Acid-Specific 9-O-Acetylesterase
Cellular Localisation Lysosome
Secreted
Alternative Antibody Names Anti-Sialate O-Acetylesterase antibody
Anti-H-Lse antibody
Anti-Sialic Acid-Specific 9-O-Acetylesterase antibody
Anti-SIAE antibody
Anti-YSG2 antibody

Information sourced from Uniprot.org

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