• Western blot analysis of extracts of various cell lines, using SLAMF7 antibody (STJ116079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SLAMF7 antibody (23-226) (STJ116079)

SKU:
STJ116079

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLAMF7 (23-226) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLAMF7
Gene ID: 57823
Uniprot ID: SLAF7_HUMAN
Immunogen Region: 23-226
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-226 of human SLAMF7 (NP_067004.3).
Immunogen Sequence: SGPVKELVGSVGGAVTFPLK SKVKQVDSIVWTFNTTPLVT IQPEGGTIIVTQNRNRERVD FPDGGYSLKLSKLKKNDSGI YYVGIYSSSLQQPSTQEYVL HVYEHLSKPKVTMGLQSNKN GTCVTNLTCCMEHGEEDVIY TWKALGQAANESHNGSILPI SWRWGESDMTFICVARNPVS RNFSSPILARKLCEGAADDP DSSM
Tissue Specificity Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. Expressed in monocytes. Isoform 3 is expressed at much lower level than isoform 1.
Function Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells. May play a role in lymphocyte adhesion. In LPS-activated monocytes negatively regulates production of pro-inflammatory cytokines. Isoform 3 does not mediate any NK cell activation.
Protein Name Slam Family Member 7
Cd2 Subset 1
Cd2-Like Receptor-Activating Cytotoxic Cells
Cracc
Membrane Protein Foap-12
Novel Ly9
Protein 19a
Cd Antigen Cd319
Database Links Reactome: R-HSA-198933
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Slam Family Member 7 antibody
Anti-Cd2 Subset 1 antibody
Anti-Cd2-Like Receptor-Activating Cytotoxic Cells antibody
Anti-Cracc antibody
Anti-Membrane Protein Foap-12 antibody
Anti-Novel Ly9 antibody
Anti-Protein 19a antibody
Anti-Cd Antigen Cd319 antibody
Anti-SLAMF7 antibody
Anti-CS1 antibody
Anti-UNQ576 antibody
Anti-PRO1138 antibody

Information sourced from Uniprot.org

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