• Western blot analysis of extracts of various cell lines, using SLC18A3 Polyclonal Antibody (STJ118508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded rat brain using SLC18A3 antibody (STJ118508) at dilution of 1:200 (40x lens).
  • Immunofluorescence analysis of mouse brain using SLC18A3 rabbit polyclonal antibody (STJ118508) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SLC18A3 antibody (473-532) (STJ118508)

SKU:
STJ118508

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Host: Rabbit
Applications: IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC18A3 (473-532) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC18A3
Gene ID: 6572
Uniprot ID: VACHT_HUMAN
Immunogen Region: 473-532
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 473-532 of human SLC18A3 (NP_003046.2).
Immunogen Sequence: GLLTRSRSERDVLLDEPPQG LYDAVRLRERPVSGQDGEPR SPPGPFDACEDDYNYYYTRS
Tissue Specificity Peripheral and central cholinergic nervous systems.
Function Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis. Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern. Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation. Can transport serotonin.
Protein Name Vesicular Acetylcholine Transporter
Vacht
Solute Carrier Family 18 Member 3
Database Links Reactome: R-HSA-264642
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Vesicular Acetylcholine Transporter antibody
Anti-Vacht antibody
Anti-Solute Carrier Family 18 Member 3 antibody
Anti-SLC18A3 antibody
Anti-VACHT antibody

Information sourced from Uniprot.org

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