• Western blot analysis of extracts of various cell lines, using SLC28A3 antibody (STJ112358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SLC28A3 antibody (612-691) (STJ112358)

SKU:
STJ112358

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC28A3 (612-691) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC28A3
Gene ID: 64078
Uniprot ID: S28A3_HUMAN
Immunogen Region: 612-691
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 612-691 of human SLC28A3 (NP_071410.1).
Immunogen Sequence: LSSTPVDINCHHVLENAFNS TFPGNTTKVIACCQSLLSST VAKGPGEVIPGGNHSLYSLK GCCTLLNPSTFNCNGISNTF
Tissue Specificity Expressed in pancreas, bone marrow, trachea, mammary gland, liver, prostate, and regions of intestine, brain, lung, placenta, testis, kidney, and heart.
Function Sodium-dependent, pyrimidine- and purine-selective. Involved in the homeostasis of endogenous nucleosides. Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine). Employs a 2:1 sodium/nucleoside ratio. Transports uridine. Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine.
Protein Name Solute Carrier Family 28 Member 3
Concentrative Na(+-Nucleoside Cotransporter 3
Cnt 3
Hcnt3
Database Links Reactome: R-HSA-83936
Reactome: R-HSA-9748787
Reactome: R-HSA-9755088
Cellular Localisation Isoform 1: Cell Membrane
Multi-Pass Membrane Protein
Isoform 2: Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Solute Carrier Family 28 Member 3 antibody
Anti-Concentrative Na(+-Nucleoside Cotransporter 3 antibody
Anti-Cnt 3 antibody
Anti-Hcnt3 antibody
Anti-SLC28A3 antibody
Anti-CNT3 antibody

Information sourced from Uniprot.org

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