• Western blot analysis of extracts of various cell lines, using Dopamine Transporter/SLC6A3 antibody (STJ117430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Dopamine Transporter/SLC6A3 antibody (STJ117430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded rat brain using Dopamine Transporter/SLC6A3 antibody (STJ117430) at dilution of 1:200 (40x lens).
  • Immunofluorescence analysis of rat brain using Dopamine Transporter/SLC6A3 rabbit polyclonal antibody (STJ117430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using Dopamine Transporter/SLC6A3 rabbit polyclonal antibody (STJ117430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SLC6A3 antibody (1-100) (STJ117430)

SKU:
STJ117430

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC6A3 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC6A3
Gene ID: 6531
Uniprot ID: SC6A3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Dopamine Transporter/SLC6A3 (NP_001035.1).
Immunogen Sequence: MSKSKCSVGLMSSVVAPAKE PNAVGPKEVELILVKEQNGV QLTSSTLTNPRQSPVEAQDR ETWGKKIDFLLSVIGFAVDL ANVWRFPYLCYKNGGGAFLV
Tissue Specificity Highly expressed in substantia nigra. Expressed in axonal varicosities in dopaminergic nerve terminals (at protein level). Expressed in the striatum (at protein level).
Function Mediates sodium- and chloride-dependent transport of dopamine. Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling.
Protein Name Sodium-Dependent Dopamine Transporter
Da Transporter
Dat
Solute Carrier Family 6 Member 3
Database Links Reactome: R-HSA-379401
Reactome: R-HSA-442660
Reactome: R-HSA-5619081
Reactome: R-HSA-5660724
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Neuron Projection
Axon
Localizes To Neurite Tips In Neuronal Cells
Colocalizes With Septin4 At Axon Terminals
Especially At The Varicosities
Alternative Antibody Names Anti-Sodium-Dependent Dopamine Transporter antibody
Anti-Da Transporter antibody
Anti-Dat antibody
Anti-Solute Carrier Family 6 Member 3 antibody
Anti-SLC6A3 antibody
Anti-DAT1 antibody

Information sourced from Uniprot.org

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