• Western blot analysis of extracts of various cell lines, using SMYD1 antibody (STJ29447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SMYD1 antibody (1-350) (STJ29447)

SKU:
STJ29447

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SMYD1 (1-350) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMYD1
Gene ID: 150572
Uniprot ID: SMYD1_HUMAN
Immunogen Region: 1-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human SMYD1 (NP_938015.1).
Immunogen Sequence: MTIGRMENVEVFTAEGKGRG LKATKEFWAADIIFAERAYS AVVFDSLVNFVCHTCFKRQE KLHRCGQCKFAHYCDRTCQK DAWLNHKNECSAIKRYGKVP NENIRLAARIMWRVEREGTG LTEGCLVSVDDLQNHVEHFG EEEQKDLRVDVDTFLQYWPP QSQQFSMQYISHIFGVINCN GFTLSDQRGLQAVGVGIFPN LGLVNHDCWPNCTVIFNNGN HEAVKSMFHTQMRIELRAL
Tissue Specificity Expression seems mostly restricted to heart and skeletal muscle.
Function Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
Protein Name Histone-Lysine N-Methyltransferase Smyd1
Set And Mynd Domain-Containing Protein 1
Database Links Reactome: R-HSA-9733709
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Smyd1 antibody
Anti-Set And Mynd Domain-Containing Protein 1 antibody
Anti-SMYD1 antibody

Information sourced from Uniprot.org

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