• Western blot analysis of extracts of various cell lines, using SPA17 antibody (STJ25667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPA17 antibody (1-151) (STJ25667)

SKU:
STJ25667

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPA17 (1-151) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPA17
Gene ID: 53340
Uniprot ID: SP17_HUMAN
Immunogen Region: 1-151
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human SPA17 (NP_059121.1).
Immunogen Sequence: MSIPFSNTHYRIPQGFGNLL EGLTREILREQPDNIPAFAA AYFESLLEKREKTNFDPAEW GSKVEDRFYNNHAFEEQEPP EKSDPKQEESQISGKEEETS VTILDSSEEDKEKEEVAAVK IQAAFRGHIAREEAKKMKTN SLQNEEKEENK
Tissue Specificity Testis and sperm specific.
Function Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.
Protein Name Sperm Surface Protein Sp17
Cancer/Testis Antigen 22
Ct22
Sp17-1
Sperm Autoantigenic Protein 17
Sperm Protein 17
Cellular Localisation Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Sperm Surface Protein Sp17 antibody
Anti-Cancer/Testis Antigen 22 antibody
Anti-Ct22 antibody
Anti-Sp17-1 antibody
Anti-Sperm Autoantigenic Protein 17 antibody
Anti-Sperm Protein 17 antibody
Anti-SPA17 antibody
Anti-SP17 antibody

Information sourced from Uniprot.org

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