• Western blot analysis of various lysates using SPON1 Rabbit polyclonal antibody (STJ117324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPON1 antibody (29-300) (STJ117324)

SKU:
STJ117324

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPON1 (29-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPON1
Gene ID: 10418
Uniprot ID: SPON1_HUMAN
Immunogen Region: 29-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human SPON1 (NP_006099.2).
Immunogen Sequence: FSDETLDKVPKSEGYCSRIL RAQGTRREGYTEFSLRVEGD PDFYKPGTSYRVTLSAAPPS YFRGFTLIALRENREGDKEE DHAGTFQIIDEEETQFMSNC PVAVTESTPRRRTRIQVFWI APPAGTGCVILKASIVQKRI IYFQDEGSLTKKLCEQDSTF DGVTDKPILDCCACGTAKYR LTFYGNWSEKTHPKDYPRRA NHWSAIIGGSHSKNYVLWEY GGYASEGVKQVAELGSPVK
Tissue Specificity Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.
Function Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.
Protein Name Spondin-1
F-Spondin
Vascular Smooth Muscle Cell Growth-Promoting Factor
Database Links Reactome: R-HSA-5083635
Reactome: R-HSA-5173214
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Spondin-1 antibody
Anti-F-Spondin antibody
Anti-Vascular Smooth Muscle Cell Growth-Promoting Factor antibody
Anti-SPON1 antibody
Anti-KIAA0762 antibody
Anti-VSGP antibody

Information sourced from Uniprot.org

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