• Western blot analysis of extracts of various cell lines, using SUMF1 antibody (STJ116516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SUMF1 antibody (34-374) (STJ116516)

SKU:
STJ116516

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUMF1 (34-374) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUMF1
Gene ID: 285362
Uniprot ID: SUMF1_HUMAN
Immunogen Region: 34-374
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-374 of human SUMF1 (NP_877437.2).
Immunogen Sequence: SQEAGTGAGAGSLAGSCGCG TPQRPGAHGSSAAAHRYSRE ANAPGPVPGERQLAHSKMVP IPAGVFTMGTDDPQIKQDGE APARRVTIDAFYMDAYEVSN TEFEKFVNSTGYLTEAEKFG DSFVFEGMLSEQVKTNIQQA VAAAPWWLPVKGANWRHPEG PDSTILHRPDHPVLHVSWND AVAYCTWAGKRLPTEAEWEY SCRGGLHNRLFPWGNKLQPK GQHYANIWQGEFPVTNTGE
Tissue Specificity Ubiquitous. Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart.
Post Translational Modifications N-glycosylated. Contains high-mannose-type oligosaccharides.
Function Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent. 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile. Known substrates include GALNS, ARSA, STS and ARSE.
Protein Name Formylglycine-Generating Enzyme
Fge
C-Alpha-Formylglycine-Generating Enzyme 1
Sulfatase-Modifying Factor 1
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-1663150
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Formylglycine-Generating Enzyme antibody
Anti-Fge antibody
Anti-C-Alpha-Formylglycine-Generating Enzyme 1 antibody
Anti-Sulfatase-Modifying Factor 1 antibody
Anti-SUMF1 antibody
Anti-PSEC0152 antibody
Anti-UNQ3037 antibody
Anti-PRO9852 antibody

Information sourced from Uniprot.org

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