• Western blot analysis of extracts of various cell lines, using TFF1/pS2 antibody (STJ115321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TFF1 antibody (25-84) (STJ115321)

SKU:
STJ115321

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TFF1 (25-84) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TFF1
Gene ID: 7031
Uniprot ID: TFF1_HUMAN
Immunogen Region: 25-84
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-84 of human TFF1/pS2 (NP_003216.1).
Immunogen Sequence: EAQTETCTVAPRERQNCGFP GVTPSQCANKGCCFDDTVRG VPWCFYPNTIDVPPEEECEF
Tissue Specificity Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).
Function Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
Protein Name Trefoil Factor 1
Breast Cancer Estrogen-Inducible Protein
Pnr-2
Polypeptide P1.A
Hp1.A
Protein Ps2
Database Links Reactome: R-HSA-9018519
Cellular Localisation Secreted
Alternative Antibody Names Anti-Trefoil Factor 1 antibody
Anti-Breast Cancer Estrogen-Inducible Protein antibody
Anti-Pnr-2 antibody
Anti-Polypeptide P1.A antibody
Anti-Hp1.A antibody
Anti-Protein Ps2 antibody
Anti-TFF1 antibody
Anti-BCEI antibody
Anti-PS2 antibody

Information sourced from Uniprot.org

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