• Western blot analysis of extracts of various cell lines, using TGM1 antibody (STJ115322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TGM1 antibody (100-280) (STJ115322)

SKU:
STJ115322

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TGM1 (100-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TGM1
Gene ID: 7051
Uniprot ID: TGM1_HUMAN
Immunogen Region: 100-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human TGM1 (NP_000350.1).
Immunogen Sequence: AGDGTIREGMLVVNGVDLLS SRSDQNRREHHTDEYEYDEL IVRRGQPFHMLLLLSRTYES SDRITLELLIGNNPEVGKGT HVIIPVGKGGSGGWKAQVVK ASGQNLNLRVHTSPNAIIGK FQFTVRTQSDAGEFQLPFDP RNEIYILFNPWCPEDIVYVD HEDWRQEYVLNESGRIYYGT E
Post Translational Modifications Palmitoylated. The membrane anchorage region possesses a cluster of five cysteines within which fatty acid(s) may become thioester-linked. It is subject to phorbol ester-stimulated phosphorylation and is hypersensitive to proteolysis, which releases the enzyme in a soluble form. Tyrosine-phosphorylated.
Function Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation.
Protein Name Protein-Glutamine Gamma-Glutamyltransferase K
Epidermal Tgase
Transglutaminase K
Tg(K
Tgk
Tgase K
Transglutaminase-1
Tgase-1
Database Links Reactome: R-HSA-6809371
Cellular Localisation Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Protein-Glutamine Gamma-Glutamyltransferase K antibody
Anti-Epidermal Tgase antibody
Anti-Transglutaminase K antibody
Anti-Tg(K antibody
Anti-Tgk antibody
Anti-Tgase K antibody
Anti-Transglutaminase-1 antibody
Anti-Tgase-1 antibody
Anti-TGM1 antibody
Anti-KTG antibody

Information sourced from Uniprot.org

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