• Western blot analysis of extracts of Raji cells, using TLR9 antibody (STJ116849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded rat spleen using TLR9 rabbit polyclonal antibody (STJ116849) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human liver using TLR9 rabbit polyclonal antibody (STJ116849) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using TLR9 rabbit polyclonal antibody (STJ116849) at dilution of 1:100 (40x lens).

Anti-TLR9 antibody (414-543) (STJ116849)

SKU:
STJ116849

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TLR9 (414-543) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLR9
Gene ID: 54106
Uniprot ID: TLR9_HUMAN
Immunogen Region: 414-543
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 414-543 of human TLR9 (NP_059138.1).
Immunogen Sequence: FPGLRYVDLSDNRISGASEL TATMGEADGGEKVWLQPGDL APAPVDTPSSEDFRPNCSTL NFTLDLSRNNLVTVQPEMFA QLSHLQCLRLSHNCISQAVN GSQFLPLTGLQVLDLSHNKL DLYHEHSFTE
Tissue Specificity Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Post Translational Modifications Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling.
Function Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production.
Protein Name Toll-Like Receptor 9
Cd Antigen Cd289
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1679131
Reactome: R-HSA-168138
Reactome: R-HSA-9679191
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975155
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Endosome
Lysosome
Cytoplasmic Vesicle
Phagosome
Relocalizes From Endoplasmic Reticulum To Endosome And Lysosome Upon Stimulation With Agonist
Exit From The Er Requires Unc93b1
Endolysosomal Localization Is Required For Proteolytic Cleavage And Subsequent Activation
Intracellular Localization Of The Active Receptor May Prevent From Responding To Self Nucleic Acid
Alternative Antibody Names Anti-Toll-Like Receptor 9 antibody
Anti-Cd Antigen Cd289 antibody
Anti-TLR9 antibody
Anti-UNQ5798 antibody
Anti-PRO19605 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance