Anti-TNF antibody (STJ140123)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification:	This antibody is epitope-affinity purified from goat antiserum.
Concentration:	3 mg/ml
Dilution Range:	WB 1:500-1:2000
Storage Instruction:	For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Gene Symbol:	TNF
Gene ID:	7124
Uniprot ID:	TNFA_HUMAN
Accession Number:	ENSG00000232810
Specificity:	Detects a band of approximately 17 kDa using Raw264.7 cell line by Western blot.
Immunogen:	Purified recombinant human TNF alpha produced in E. coli.

Post Translational Modifications	The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated.glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Tumor necrosis factor, soluble form: The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.
Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. Promotes osteoclastogenesis and therefore mediates bone resorption. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Protein Name	Tumor Necrosis Factor Cachectin Tnf-Alpha Tumor Necrosis Factor Ligand Superfamily Member 2 Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form N-Terminal Fragment Ntf - Intracellular Domain 1 Icd1 - Intracellular Domain 2 Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form
Database Links	Reactome: R-HSA-381340 Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 Reactome: R-HSA-5626978 Reactome: R-HSA-5668541 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-75893
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Tumor Necrosis Factor Membrane Form: Membrane Soluble Form: Secreted C-Domain 1: Secreted C-Domain 2: Secreted
Alternative Antibody Names	Anti-Tumor Necrosis Factor antibody Anti-Cachectin antibody Anti-Tnf-Alpha antibody Anti-Tumor Necrosis Factor Ligand Superfamily Member 2 antibody Anti-Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form antibody Anti-N-Terminal Fragment antibody Anti-Ntf - Intracellular Domain 1 antibody Anti-Icd1 - Intracellular Domain 2 antibody Anti-Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form antibody Anti-TNF antibody Anti-TNFA antibody Anti-TNFSF2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance