Primary antibodies

 

Loading control antibodies

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Primary Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading control antibodies

Loading control antibodies

Loading control antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit monoclonal antibodies

Rabbit monoclonal antibodies

Rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho antibodies

Phospho antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout validated primary antibodies

KO-validated primary antibodies

        In a knockout validation, antibody specificity is confirmed by testing the primary antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat primary antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

Isotype

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Clonality

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  • Anti-Beta Actin antibody [STJ91464]

    Western blot (WB) analysis of beta actin antibody with Lane 1: Hela, Lane 2: Jurkat cells. Membrane was blocked with skimmed milk, and probed with primary antibody at 1:1000.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC-p, WB
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  • Anti-Beta-Actin antibody [STJ99028]

    Western blot detection of beta actin in C6, 3T3, COS7, CHO-k1, K562 and Goat muscle cell lysates using beta actin mouse mAb (1:10000 diluted). Predicted band size: 45KDa. Observed band size: 45KDa.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Arabidopsis, Chlamydomonas, Cucumber, D.melanogaster, Goat, Hamster, Human, Monkey, Mouse, O.sativa, PichiaPastoris, Rabbit, Rat, S.Cerevisiae, Zebrafish
    Applications: WB
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  • Anti-Beta-Tubulin antibody [STJ99132]

    Western blot detection of Tubulin beta in COS7, LNCap, MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted). Predicted band size: 55KDa. Observed band size: 55KDa.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Chlamydomonas, Goat, Hamster, Human, Monkey, Mouse, Rat
    Applications: IF, WB
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  • Anti-GAPDH antibody [STJ99066]

    Western blot detection of GAPDH in Hela, 3T3, C6, COS7, CHO-K1, Rat brain and Jurkat cell lysates using GAPDH mouse mAb (1:5000 diluted). Predicted band size: 36KDa. Observed band size: 36KDa.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Hamster, Human, Monkey, Mouse, PichiaPastoris, Rat, S.Cerevisiae
    Applications: WB
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  • Anti-PCNA antibody [STJ99203]

    Western blot detection of PCNA in Hela, 3T3, COS7, C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted). Predicted band size: 36KDa. Observed band size: 36KDa.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Hamster, Human, Monkey, Mouse, Rat
    Applications: ICC, IHC, IP, WB
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  • Anti-GAPDH (Human Specific) antibody [STJ99065]

    Western blot detection of GAPDH (human specific) in SW480, A431, K562, Jurkat and Hela cell lysates using GAPDH (human specific) mouse mAb (1:5000 diluted). Predicted band size: 37KDa. Observed band size: 37KDa.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Human, Monkey
    Applications: WB
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  • Anti-Actinin-Alpha antibody [STJ98460]

    Western Blot (WB) analysis using Actinin-alpha2 Monoclonal Antibody against HepG2 cell lysate.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Bovine, Chicken, Dog, Human, Pig, Rat, Zebrafish
    Applications: IF, WB
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  • Anti-PRL Antibody [STJ502655]

    Immunohistochemistry of human tissue with Anti-PRL primary antibody (STJ502655) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity:
    Applications: WB
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  • Anti-PRL Antibody [STJ502654]

    Immunohistochemistry of human tissue with Anti-PRL primary antibody (STJ502654) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 5X.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity:
    Applications: WB
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  • Anti-GAPDH Antibody [STJ501277]

    Immunohistochemistry of mouse tissue with Anti-GAPDH primary antibody (STJ501277) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity:
    Applications: IP, WB
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  • Anti-ACTN4 Antibody [STJ500045]

    Western blot with Anti-ACTN4 Antibody (STJ500045) of 1) rat podocyte membranes and 2) NIH3TS3 cells. Antibody was diluted 1:500 in buffer.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity:
    Applications: IP, WB
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  • Anti-ACTB Antibody [STJ500039]

    Immunohistochemistry with Anti-ACTB Antibody (STJ500039) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Right slide is negative control.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity:
    Applications: IP, WB
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