Primary antibodies

 

Loading control antibodies

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Primary Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading control antibodies

Loading control antibodies

Loading control antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit monoclonal antibodies

Rabbit monoclonal antibodies

Rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho antibodies

Phospho antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout validated primary antibodies

KO-validated primary antibodies

        In a knockout validation, antibody specificity is confirmed by testing the primary antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat primary antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-GAPDH antibody [STJ96931]

    Western blot (WB) analysis of Hela, rat brain, rabbit muscle, sheep muscle and mouse brain cells, using monoclonal Anti-GAPDH Antibody (STJ96931) at 1:10000.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Bovine, Canine, Hamster, Human, Insect, Mouse, Rabbit, Rat, Sheep, Simian, Swine, Yeast
    Applications: IF, IHC, WB
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  • Anti-CTTN Antibody [STJ70093]

    STJ70093 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat
    Applications: ELISA, WB
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  • Anti-RHEB antibody [STJ98588]

    Western Blot (WB) analysis of Mouse Kidney Mouse Brain cells using RHEB Polyclonal Antibody diluted at 1:2000.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Bovine, Human, Mouse, Rat
    Applications: ELISA, WB
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  • Anti-ZO1 antibody [STJ140055]

    Immunofluorescence was carried out on primary RPE cells, using Anti-ZO1 polyclonal antibody at a 1:100 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian, Swine
    Applications: IF, IHC, WB
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  • Anti-CD63 antibody [STJ140029]

    Immunofluorescence was carried out on 4% PFA fixed Hepa1-6 cells, using Anti-CD63 polyclonal antibody at a 1:50 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian
    Applications: IF, WB
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  • Anti-beta-Actin antibody [STJ140081]

    Immunofluorescence was carried out on 0.1% saponin permeabilized and methanol fixed NHI/3T3 cells, using anti-beta-Actin polyclonal antibody at a 1:100 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian
    Applications: IF, WB
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  • Anti-CD19 antibody [STJ140025]

    40-100 ng of pepsin was isolated in porcine gastric mucosa and treated with Anti-Pepsin antibody which was diluted at 1:500, and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian
    Applications: WB
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  • Anti-GFAP antibody [STJ140132]

    Western Blot was carried out using Anti-GFAP polyclonal antibody at a 1:2,500 dilution, with lysates of 50 µg per lane and secondary anti-goat IgG (HRP) at 1:10,000 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian
    Applications: WB
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  • Anti-CDH1 antibody [STJ140099]

    Western Blot was carried out on HeLa and COS7 cells using Anti-CDH1 polyclonal at a 1:500 dilution, with lysates of 100 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian
    Applications: IF, IHC, WB
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  • Anti-Rab25 antibody [STJ140073]

    Immunofluorescence was carried out on methanol fixed Hepa1-6 cells, using Anti-Rab25 polyclonal antibody at a 1:50 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian
    Applications: IF, IHC, WB
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  • Anti-Rab8 antibody [STJ140064]

    Immunofluorescence was carried out on 4% PFA fixed primary macrophages, using Anti-Rab8 polyclonal antibody at a 1:100 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian
    Applications: IF, IHC, WB
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  • Anti-Rab5 antibody [STJ140059]

    Immunofluorescence was carried out on 4% PFA fixed B6-RPE07 cells, using Anti-Rab5 polyclonal antibody at a 1:50 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian
    Applications: IF, IHC, WB
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