Primary antibodies

 

Loading control antibodies

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Primary Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading control antibodies

Loading control antibodies

Loading control antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit monoclonal antibodies

Rabbit monoclonal antibodies

Rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho antibodies

Phospho antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout validated primary antibodies

KO-validated primary antibodies

        In a knockout validation, antibody specificity is confirmed by testing the primary antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat primary antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-GAPDH antibody [STJ96931]

    Western blot (WB) analysis of Hela, rat brain, rabbit muscle, sheep muscle and mouse brain cells, using monoclonal Anti-GAPDH Antibody (STJ96931) at 1:10000.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Chimpanzee, Dog, Hamster, Human, Insect, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast
    Applications: IF, IHC-p, WB
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  • Anti-Beta actin antibody [STJ96941]

    Western blot of TRF2-sh2 NPC lysate with Anti-beta actin antibody at 1:5000, with single specific band from beta actin.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Chimpanzee, Dog, Hamster, Human, Monkey, Mouse, Rabbit, Rat
    Applications: WB
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  • Anti-Beta-actin antibody [STJ96930]

    Western blot (WB) analysis of beta-actin monoclonal antibody.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Chimpanzee, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat
    Applications: IF, IHC-p, WB
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  • Anti-Cortactin/EMS1 antibody [STJ70093]

    STJ70093 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Cow, Dog, Human, Mouse, Rat
    Applications: ELISA, WB
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  • Anti-Beta-actin antibody [STJ97040]

    Western Blot (WB) analysis of 1) 293T, 2) Mouse Kidney tissue, 3) HeLa, 4) Rat Heart tissue, 5) Rat Brain tissue, diluted at 1:5000.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Chimpanzee, Dog, Hamster, Human, Monkey, Mouse, Rabbit, Rat
    Applications: IHC-p, WB
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  • Anti-BDNF antibody [STJ73299]

    STJ73299 (1µg/ml) staining of PFA-perfused cryosection of Mouse sciatic nerve (left) and injured sciatic nerve (right). Alexa 488-staining. Data obtained from Simon Glerup, Department of Biomedicine, Aarhus University, Denmark.This data is from a
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Dog, Human, Mouse, Rat
    Applications: ELISA, IF
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  • Anti-Beta-actin antibody [STJ97035]

    Western Blot (WB) analysis of 1) HeLa, 2) Mouse Brain tissue, 3) Rat Brain tissue, diluted at 1:5000.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Dog, Human, Monkey, Mouse, Rat
    Applications: WB
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  • Anti-Atrial natriuretic factor antibody [STJ72680]

    STJ72680 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Cow, Dog, Human, Mouse, Pig, Rat
    Applications: ELISA, IHC, WB
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  • Anti-PRDM1/MEL1 antibody [STJ72074]

    STJ72074 (3.8µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Cow, Dog, Human, Mouse, Pig, Rat
    Applications: ELISA, FC, IF, WB
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  • Anti-RNASEN/Drosha antibody [STJ71047]

    STJ71047 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Dog, Human, Mouse, Rat
    Applications: ELISA, WB
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  • Anti-AIF1/IBA1 antibody [STJ70888]

    STJ70888 (2µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Dog, Human, Mouse
    Applications: ELISA, FC, IF, IHC, WB
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  • Anti-Androgen Receptor antibody [STJ70576]

    STJ70576 (2µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Cow, Dog, Human, Mouse, Pig, Rat
    Applications: ChIP, ELISA, IF, IHC, WB
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