Anti-ADAM10 antibody

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Product name Anti-ADAM10 antibody
Short Description Rabbit polyclonal against ADAM10
Description Rabbit polyclonal to ADAM10.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:20000
Specificity ADAM10 Polyclonal Antibody detects endogenous levels of ADAM10 protein.
Protein Name Disintegrin and metalloproteinase domain-containing protein 10
Kuzbanian protein homolog
Mammalian disintegrin-metalloprotease
CD antigen CD156c
Immunogen Synthesized peptide derived from human ADAM10
Immunogen Region 170-250 aa, Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 102
Gene Symbol ADAM10
Molecular Weight 85 kDa
Database Links HGNC:188
Alternative Names Disintegrin and metalloproteinase domain-containing protein 10
Kuzbanian protein homolog
Mammalian disintegrin-metalloprotease
CD antigen CD156c
Function Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface . Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) . Contributes to the normal cleavage of the cellular prion protein . Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity . Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis . Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form . Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B . May regulate the EFNA5-EPHA3 signaling .
Post-translational Modifications The precursor is cleaved by a furin endopeptidase.
Cellular Localization Cell membrane Golgi apparatus membrane. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
Tissue Specificity Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
Sequence and Domain Family The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.; The Cys-rich region C-terminal to the disintegrin domain functions as a substrate-recognition module, it recognizes the EFNA5-EPHA3 Complex but not the individual proteins . Both Cys-rich and stalk region are necessary for interaction with TSPAN5, TSPAN10, TSPAN14, TSPAN17, TSPAN33 . Stalk region is sufficient for interaction with TSPAN15 .
Swiss-Prot Key O14672_HUMAN
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