Anti-Bcl-2 antibody (KLH)

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Product name Anti-Bcl-2 antibody (KLH)
Short Description Mouse monoclonal against Bcl-2 (KLH)
Description Mouse monoclonal to Bcl-2 (KLH).
Applications ELISA, FC, IF, IHC
Dilution range IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Specificity Bcl-2 Monoclonal Antibody detects endogenous levels of Bcl-2 protein.
Protein Name Apoptosis regulator Bcl-2
Immunogen Synthetic peptide corresponding to residues surrounding Bcl-2, conjugated to KLH.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application FC IF IHC
Host Mouse
Clonality Monoclonal
Clone ID 8E+12
Reactivity Human, Mouse
Conjugation KLH
Purification Affinity purification
Isotype IgG1
Formulation Ascitic fluid containing 0.03% sodium azide.
Gene ID 596
Gene Symbol BCL2
Database Links HGNC:990
Alternative Names Apoptosis regulator Bcl-2
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release .
Post-translational Modifications Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) . Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
Cellular Localization Mitochondrion outer membrane Nucleus membrane Endoplasmic reticulum membrane
Tissue Specificity Expressed in a variety of tissues.
Sequence and Domain Family BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity. The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.; The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
Swiss-Prot Key P10415_HUMAN
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