Anti-beta III Tubulin antibody

[STJ97123] Download PDF Print Data Sheet

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Product name Anti-beta III Tubulin antibody
Short Description Rabbit polyclonal against beta III Tubulin
Description Beta III tubulin is a protein encoded by the tubb3 gene which is approximately 50,4 kDa. Beta III tubulin is localised to the cytoplasm and cytoskeleton. It is involved in signalling within gap junctions, development slit-robo signalling, sertoli-sertoli cell junction dynamics, chaperonin-mediated protein folding and tubulin folding. This protein falls under the one of two protein families, namely beta and alpha tubulin and is one of six beta tubulin isoforms. Beta III tubulin is expressed in the neurons of the peripheral and central nervous system and plays an important role in guidance and maintenance and axons. Mutations in the tubb3 gene result in congenital fibrosis of extraocular muscles type. STJ97123 was developed from clone Mix. It was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta III tubulin protein.
Applications FC, IF, WB
Dilution range WB 1:2000-5000
Specificity The antibody detects endogenous beta III tubulin protein.
Protein Name Tubulin beta-3 chain
Tubulin beta-4 chain
Tubulin beta-III
Immunogen Synthetic Peptide
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 10381
Gene Symbol TUBB3
Database Links HGNC:20772
Alternative Names Tubulin beta-3 chain
Tubulin beta-4 chain
Tubulin beta-III
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Post-translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group . Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold . Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Cellular Localization Cytoplasm, cytoskeleton.
Tissue Specificity Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
Sequence and Domain Family The highly acidic C-terminal region may bind cations such as calcium.
Swiss-Prot Key Q13509_HUMAN
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Ask us a question

I am interested in this antibody in 0.3mg size, 1mg/mL. Would it be possible to change the storage buffer to PBS and not include sodium azide?
Question by: Guest on 11 Apr 2018 12:40:00
Hello, thank you for your query. If you would like to place a custom order, the product should be at least 1 mg. There will be no additional cost for changing the storage buffer. Please contact us at [email protected] for further information.
Answer by: Customer Support on 11 Apr 2018 12:43:00


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