Anti-Cofilin antibody

[STJ92371] Download PDF Print Data Sheet

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Product name Anti-Cofilin antibody
Short Description Rabbit polyclonal against Cofilin
Description Cofilin is a protein encoded by the JAK1 gene which is approximately 133,2 kDa. Cofilin is localised to the endomembrane system and is involved in RET signalling, common cytokine receptor gamma-chain family signalling pathways, Th17 cell differentiation and IL27-mediated signalling events. It phosphorylates STAT proteins and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Cofilin is expressed at higher levels in primary colon tumor than normal colon tissue. Mutations in the JAK1 gene may result in mixed lacrimal gland cancer, t-cell prolymphocytic leukemia and chilblain lupus. The antibody STJ92371 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Cofilin protein.
Applications ELISA, IF, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity Cofilin Polyclonal Antibody detects endogenous levels of Cofilin protein.
Protein Name Cofilin-1
18 kDa phosphoprotein
Cofilin, non-muscle isoform
Immunogen Synthesized peptide derived from human Cofilin around the non-phosphorylation site of S3.
Immunogen Region The antiserum was produced against synthesized peptide derived from human Cofilin. AA range:1-50
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1072
Gene Symbol CFL1
Molecular Weight 19 kDa
Database Links HGNC:1874
Alternative Names Cofilin-1
18 kDa phosphoprotein
Cofilin, non-muscle isoform
Function Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation . Required for neural tube morphogenesis and neural crest cell migration .
Post-translational Modifications Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells . Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal . Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.
Cellular Localization Nucleus matrix Cytoplasm, cytoskeleton Cell projection, ruffle membrane Cell projection, lamellipodium membrane. Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Tissue Specificity Widely distributed in various tissues.
Swiss-Prot Key P23528_HUMAN
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